Identifikasi senyawa steroid dalam tumbuhan

The  aims  of  this  research was to identify the flavonoid content in binahong tuber and determine the effect of 70 % ethanol extract of binahong tuber on blood glucose level and SOD activity in rats ( Rattus norvegicus ) induced alloxan. The extract was maceration and then used to test the classes of its compound content using phytochemical test, the flavonoid compound contained in the extract was separated by analytic thin layer chromatography (TLC) and antidiabetic effect against rats induced alloxan with dose of alloxan was 32 mg / 200 g BW. The results showed that the 70 % ethanol extract of binahong tuber contains flavonoids, alkaloids, tannins, saponins and terpenoids. Butanol: acetic acid: water (4: 1: 5) was the best eluent for separation of flavonoid compound in extract and binahong tuber extracts treatment with variation of extract dose, 25, 60 and 75 mg/kg BW, reduced the blood glucose levels and increased the SOD ( Superoxide dismutase ) activity of rat, that are 5,511 ; 5,217 and 4,942 u/mL respectively.

Identifikasi senyawa steroid dalam tumbuhan

identifikasi senyawa steroid dalam tumbuhan

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