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Tie off the upper arm with the rubber tourniquet or use the cuff attach 5-10 cc syringe with normal saline to the Butterfly needle and insert into the most visible vein in the anticubital fossa inject 2-3 cc of saline to see that the needle is well into the vein. Secure the Butterfly needle with silk tape. Withdraw cc of Heparin sodium into the 60 cc syringe. Turn on the ozone generator with oxygen only to purge the system. Turn on ozone generator to produce ozone. Insert tip of syringe into outlet of ozone generator and draw in 30 cc of ozone. Put a No. 25 needle on the syringe. Insert the Butterfly needle into the vein and open the tourniquet the saline syringe and attach the 60 cc syringe with ozone in it. Retie the tourniquet and then slowly fill the syringe to make the blood mix with the ozone. The blood will turn bright red. Untie the tourniquet and then slowly inject the blood over 5 minutes. Remove the 60 cc syringe from the tubing to the Butterfly needle and re-attach the 5-10 cc syringe of saline inject 5 cc of saline to wash the blood back through the needle. If desired, repeat twice with further injections of Heparin and ozone. Remove butterfly needle and apply cotton ball to injection site.